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In vitro evaluation of wound healing enhancement effects of the integrated device. (a) Schematic representation of PDRN delivery via iontophoresis into the culture medium. (b) Fluorescence images of HDK (HaCaT) cells from different experimental groups captured at indicated time points. (c) Cell proliferation ratios of HDK cells from each group assessed at indicated time points. (d) Fluorescence images of HDF <t>(CCD‐986Sk)</t> cells and (e) corresponding proliferation ratios from each experimental group recorded at indicated time points. (f) Microscopic images showing scratch assays of HDK cells for each group at specific time intervals. (g) Quantitative analysis of scratch areas in HDK cells from each group across indicated time intervals. (h) Microscopic images of scratch assays performed on HDF cells and i) corresponding quantitative analysis of scratch areas from each group over time. Data represent means ± SD; n = 5. Statistical analysis was performed using one‐way ANOVA with Bonferroni‐corrected post‐hoc t ‐tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.
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In vitro evaluation of wound healing enhancement effects of the integrated device. (a) Schematic representation of PDRN delivery via iontophoresis into the culture medium. (b) Fluorescence images of HDK (HaCaT) cells from different experimental groups captured at indicated time points. (c) Cell proliferation ratios of HDK cells from each group assessed at indicated time points. (d) Fluorescence images of HDF <t>(CCD‐986Sk)</t> cells and (e) corresponding proliferation ratios from each experimental group recorded at indicated time points. (f) Microscopic images showing scratch assays of HDK cells for each group at specific time intervals. (g) Quantitative analysis of scratch areas in HDK cells from each group across indicated time intervals. (h) Microscopic images of scratch assays performed on HDF cells and i) corresponding quantitative analysis of scratch areas from each group over time. Data represent means ± SD; n = 5. Statistical analysis was performed using one‐way ANOVA with Bonferroni‐corrected post‐hoc t ‐tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.
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In vitro evaluation of wound healing enhancement effects of the integrated device. (a) Schematic representation of PDRN delivery via iontophoresis into the culture medium. (b) Fluorescence images of HDK (HaCaT) cells from different experimental groups captured at indicated time points. (c) Cell proliferation ratios of HDK cells from each group assessed at indicated time points. (d) Fluorescence images of HDF (CCD‐986Sk) cells and (e) corresponding proliferation ratios from each experimental group recorded at indicated time points. (f) Microscopic images showing scratch assays of HDK cells for each group at specific time intervals. (g) Quantitative analysis of scratch areas in HDK cells from each group across indicated time intervals. (h) Microscopic images of scratch assays performed on HDF cells and i) corresponding quantitative analysis of scratch areas from each group over time. Data represent means ± SD; n = 5. Statistical analysis was performed using one‐way ANOVA with Bonferroni‐corrected post‐hoc t ‐tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Healthcare Materials

Article Title: Skin‐Interfaced Therapeutic Patches for Wound Fluid Management and Transdermal Drug Delivery

doi: 10.1002/adhm.202504450

Figure Lengend Snippet: In vitro evaluation of wound healing enhancement effects of the integrated device. (a) Schematic representation of PDRN delivery via iontophoresis into the culture medium. (b) Fluorescence images of HDK (HaCaT) cells from different experimental groups captured at indicated time points. (c) Cell proliferation ratios of HDK cells from each group assessed at indicated time points. (d) Fluorescence images of HDF (CCD‐986Sk) cells and (e) corresponding proliferation ratios from each experimental group recorded at indicated time points. (f) Microscopic images showing scratch assays of HDK cells for each group at specific time intervals. (g) Quantitative analysis of scratch areas in HDK cells from each group across indicated time intervals. (h) Microscopic images of scratch assays performed on HDF cells and i) corresponding quantitative analysis of scratch areas from each group over time. Data represent means ± SD; n = 5. Statistical analysis was performed using one‐way ANOVA with Bonferroni‐corrected post‐hoc t ‐tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The HDF cell line CCD‐986Sk (RRID: CVCL_4200) was obtained from the Korean Cell Line Bank (KCLB, Seoul, South Korea) on August 2024 and was likewise confirmed to be free of contamination upon arrival.

Techniques: In Vitro, Fluorescence